Primers Available
Organism: Sebastiscus marmoratus mitochondrion
Repeat:(T)10
Start:16169 End:16178
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAGCCTTCTTTCCATAGGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTTTAACGCGAAAAAGCCAA | |||||||||||||
| Tm_F_1 | 60.173 | Size_F_1 | 20 | Tm_R_1 | 60.239 | Size_R_1 | 20 | PRODUCT Size | 238 bp | Primer Start | 16136 | Primer End | 16373 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGGGGTTTCACAAAATGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCATAGGATGATTCCCACC | |||||||||||||
| Tm_F_2 | 60.029 | Size_F_2 | 20 | Tm_R_2 | 59.563 | Size_R_2 | 20 | PRODUCT Size | 243 bp | Primer Start | 15999 | Primer End | 16241 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCCATACGTTCAGCCTAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCATAGGATGATTCCCACC | |||||||||||||
| Tm_F_3 | 59.955 | Size_F_3 | 20 | Tm_R_3 | 59.563 | Size_R_3 | 20 | PRODUCT Size | 176 bp | Primer Start | 16066 | Primer End | 16241 |