Primers Available
Organism: Microcosmus sulcatus mitochondrion
Repeat:(TTA)4
Start:2477 End:2488
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTCGGCAATAATTGTGTCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTCCCACTCCCTCTCACCT | |||||||||||||
| Tm_F_1 | 58.912 | Size_F_1 | 21 | Tm_R_1 | 59.581 | Size_R_1 | 20 | PRODUCT Size | 175 bp | Primer Start | 2396 | Primer End | 2570 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTCGGCAATAATTGTGTCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCACTGCCTGCATACTGCC | |||||||||||||
| Tm_F_2 | 58.912 | Size_F_2 | 21 | Tm_R_2 | 60.465 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 2396 | Primer End | 2642 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTCGGCAATAATTGTGTCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGCATCTCTCCGCCTAAAT | |||||||||||||
| Tm_F_3 | 58.912 | Size_F_3 | 21 | Tm_R_3 | 60.601 | Size_R_3 | 20 | PRODUCT Size | 222 bp | Primer Start | 2396 | Primer End | 2617 |