Primers Available
Organism: Radopholus similis mitochondrion
Repeat:(TTTA)3attattaataattttaattatt(TTA)5
Start:5729 End:5777
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGTCGTAAGGTAGAGTTGTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTAAAGACATAAATCCACTTTCAAA | |||||||||||||
| Tm_F_1 | 57.164 | Size_F_1 | 22 | Tm_R_1 | 57.419 | Size_R_1 | 27 | PRODUCT Size | 231 bp | Primer Start | 5630 | Primer End | 5860 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGTCGTAAGGTAGAGTTGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCCTAAAGACATAAATCCACTTTCAA | |||||||||||||
| Tm_F_2 | 57.164 | Size_F_2 | 22 | Tm_R_2 | 57.419 | Size_R_2 | 27 | PRODUCT Size | 232 bp | Primer Start | 5630 | Primer End | 5861 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGTCGTAAGGTAGAGTTGTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTCCTAAAGACATAAATCCACTTTCA | |||||||||||||
| Tm_F_3 | 57.164 | Size_F_3 | 22 | Tm_R_3 | 57.419 | Size_R_3 | 27 | PRODUCT Size | 233 bp | Primer Start | 5630 | Primer End | 5862 |