Primers Available
Organism: Radopholus similis mitochondrion
Repeat:(T)13
Start:1539 End:1551
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGTTCTTCTAGACAAGCACTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTCACTCTCACCCTCAGAA | |||||||||||||
| Tm_F_1 | 57.676 | Size_F_1 | 22 | Tm_R_1 | 57.315 | Size_R_1 | 20 | PRODUCT Size | 200 bp | Primer Start | 1420 | Primer End | 1619 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGTTCTTCTAGACAAGCACTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACTCACTCTCACCCTCAGA | |||||||||||||
| Tm_F_2 | 57.676 | Size_F_2 | 22 | Tm_R_2 | 57.315 | Size_R_2 | 20 | PRODUCT Size | 201 bp | Primer Start | 1420 | Primer End | 1620 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGTTCTTCTAGACAAGCACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTCACTCTCACCCTCAGAAA | |||||||||||||
| Tm_F_3 | 57.676 | Size_F_3 | 22 | Tm_R_3 | 58.036 | Size_R_3 | 21 | PRODUCT Size | 200 bp | Primer Start | 1420 | Primer End | 1619 |