Primers Available
Organism: Radopholus similis mitochondrion
Repeat:(AAAT)3tttaa(ATTT)3
Start:13185 End:13213
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTGTACAGATTGAGAAATTTAGTTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAAGCCTCAACATGTAATTTTGGT | |||||||||||||
| Tm_F_1 | 57.779 | Size_F_1 | 27 | Tm_R_1 | 59.696 | Size_R_1 | 25 | PRODUCT Size | 240 bp | Primer Start | 13048 | Primer End | 13287 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGTGTACAGATTGAGAAATTTAGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAAGCCTCAACATGTAATTTTGGT | |||||||||||||
| Tm_F_2 | 57.779 | Size_F_2 | 27 | Tm_R_2 | 59.696 | Size_R_2 | 25 | PRODUCT Size | 241 bp | Primer Start | 13047 | Primer End | 13287 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTGTACAGATTGAGAAATTTAGTTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTAGCTAATGTTGAAGCCTCAA | |||||||||||||
| Tm_F_3 | 57.779 | Size_F_3 | 27 | Tm_R_3 | 57.579 | Size_R_3 | 23 | PRODUCT Size | 252 bp | Primer Start | 13048 | Primer End | 13299 |