Primers Available
Organism: Sinocyclocheilus altishoulderus mitochondrion
Repeat:(TA)13
Start:807 End:832
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGCCCCCTTTGGTTTTTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTTGGTGGTGAGTTGTTGC | |||||||||||||
| Tm_F_1 | 59.89 | Size_F_1 | 20 | Tm_R_1 | 59.898 | Size_R_1 | 20 | PRODUCT Size | 202 bp | Primer Start | 666 | Primer End | 867 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCATTAGCCAACGAGTCGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTGGTGGTGAGTTGTTGC | |||||||||||||
| Tm_F_2 | 59.63 | Size_F_2 | 20 | Tm_R_2 | 59.898 | Size_R_2 | 20 | PRODUCT Size | 101 bp | Primer Start | 767 | Primer End | 867 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCATTAGCCAACGAGTCGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGACCATGCCTTTGTGCATG | |||||||||||||
| Tm_F_3 | 59.63 | Size_F_3 | 20 | Tm_R_3 | 60.109 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 767 | Primer End | 1015 |