Primers Available
Organism: Natural gynogenetic Carassius auratus red var. mitochondrion
Repeat:(TA)9
Start:807 End:824
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCCAACGAGTTGAGGTACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGACCATGCCTTTGTGCATG | |||||||||||||
| Tm_F_1 | 60.039 | Size_F_1 | 20 | Tm_R_1 | 60.109 | Size_R_1 | 20 | PRODUCT Size | 234 bp | Primer Start | 772 | Primer End | 1005 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCAACGAGTTGAGGTACGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGACCATGCCTTTGTGCATG | |||||||||||||
| Tm_F_2 | 60.039 | Size_F_2 | 20 | Tm_R_2 | 60.109 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 773 | Primer End | 1005 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGAAACCAAGGAGGACCCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGACCATGCCTTTGTGCATG | |||||||||||||
| Tm_F_3 | 59.89 | Size_F_3 | 20 | Tm_R_3 | 60.109 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 743 | Primer End | 1005 |