Primers Available
Organism: Hemidactylus frenatus mitochondrion
Repeat:(ATC)4
Start:9713 End:9724
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGGGACTGCACGACTACCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTTGGGGTGATGCCAAGAT | |||||||||||||
| Tm_F_1 | 59.961 | Size_F_1 | 20 | Tm_R_1 | 60.033 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 9563 | Primer End | 9842 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAGCACCAACAACCACAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTTGGGGTGATGCCAAGAT | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 60.033 | Size_R_2 | 20 | PRODUCT Size | 168 bp | Primer Start | 9675 | Primer End | 9842 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGACTGCACGACTACCATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTTGGGGTGATGCCAAGAT | |||||||||||||
| Tm_F_3 | 59.751 | Size_F_3 | 20 | Tm_R_3 | 60.033 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 9565 | Primer End | 9842 |