Primers Available
Organism: Hemidactylus frenatus mitochondrion
Repeat:(ACC)4
Start:13669 End:13680
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTCAAAAGAGCAGGCCAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTGTGGCTTATTGGTCA | |||||||||||||
| Tm_F_1 | 59.963 | Size_F_1 | 20 | Tm_R_1 | 59.961 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 13642 | Primer End | 13911 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTCAAAAGAGCAGGCCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGTACTCTGTTGCCTTGGCT | |||||||||||||
| Tm_F_2 | 59.963 | Size_F_2 | 20 | Tm_R_2 | 60.321 | Size_R_2 | 20 | PRODUCT Size | 181 bp | Primer Start | 13642 | Primer End | 13822 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTATACGGCACTCGCAGACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTGGGGGTTGCGTTTAGT | |||||||||||||
| Tm_F_3 | 60.318 | Size_F_3 | 20 | Tm_R_3 | 60.107 | Size_R_3 | 20 | PRODUCT Size | 223 bp | Primer Start | 13504 | Primer End | 13726 |