Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(TATC)3catatg(GATA)3
Start:69219 End:69248
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGTCGGTAGTTGCTCAGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGAGGGTGTCTCGCCTTTT | |||||||||||||
| Tm_F_1 | 60.037 | Size_F_1 | 20 | Tm_R_1 | 59.678 | Size_R_1 | 20 | PRODUCT Size | 183 bp | Primer Start | 69103 | Primer End | 69285 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACGAGGAAAGGGGCACTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGAGGGTGTCTCGCCTTTT | |||||||||||||
| Tm_F_2 | 59.815 | Size_F_2 | 20 | Tm_R_2 | 59.678 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 69068 | Primer End | 69285 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAAAGCCTCGAAGTCGGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGAGGGTGTCTCGCCTTTT | |||||||||||||
| Tm_F_3 | 60.389 | Size_F_3 | 20 | Tm_R_3 | 59.678 | Size_R_3 | 20 | PRODUCT Size | 194 bp | Primer Start | 69092 | Primer End | 69285 |