Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(T)23
Start:133339 End:133361
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAACCGCTTCCCATACTCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAAGTCAAATCAGTAGAAGGAAGA | |||||||||||||
| Tm_F_1 | 59.745 | Size_F_1 | 20 | Tm_R_1 | 57.454 | Size_R_1 | 25 | PRODUCT Size | 197 bp | Primer Start | 133287 | Primer End | 133483 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCGCTTCCCATACTCCCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAAGTCAAATCAGTAGAAGGAAGA | |||||||||||||
| Tm_F_2 | 59.736 | Size_F_2 | 20 | Tm_R_2 | 57.454 | Size_R_2 | 25 | PRODUCT Size | 195 bp | Primer Start | 133289 | Primer End | 133483 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCTACTCCACTCCGGTGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTTGTAGCATCTCAAATGATTAAGA | |||||||||||||
| Tm_F_3 | 60.034 | Size_F_3 | 20 | Tm_R_3 | 58.076 | Size_R_3 | 26 | PRODUCT Size | 260 bp | Primer Start | 133164 | Primer End | 133423 |