Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(T)13actacactaataaggagta(AT)9gttatttttgtcaaacatagtggggttgggccctaaaaaaagttctgtcatatctatttgtttcct(A)14(TA)6gatagata(T)12
Start:80161 End:80322
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTAGAAATGGTATCGTTATTCGTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTTGGGTGTCACGAAGAGA | |||||||||||||
| Tm_F_1 | 59.08 | Size_F_1 | 26 | Tm_R_1 | 58.234 | Size_R_1 | 21 | PRODUCT Size | 280 bp | Primer Start | 80085 | Primer End | 80364 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTAGAAATGGTATCGTTATTCGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTTGGGTGTCACGAAGAGA | |||||||||||||
| Tm_F_2 | 57.898 | Size_F_2 | 25 | Tm_R_2 | 58.234 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 80085 | Primer End | 80364 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGAAATGGTATCGTTATTCGTGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACATTTGGGTGTCACGAAGAGA | |||||||||||||
| Tm_F_3 | 57.163 | Size_F_3 | 25 | Tm_R_3 | 59.896 | Size_R_3 | 22 | PRODUCT Size | 278 bp | Primer Start | 80088 | Primer End | 80365 |