Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(T)13
Start:54704 End:54716
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCACTCTGTTACATGCGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTGCGTTTCATGCGGAGTG | |||||||||||||
| Tm_F_1 | 60.067 | Size_F_1 | 21 | Tm_R_1 | 59.831 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 54641 | Primer End | 54877 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACAGCGAGATGGGCAAAAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGATCGACCAAATGTCTGG | |||||||||||||
| Tm_F_2 | 60.251 | Size_F_2 | 20 | Tm_R_2 | 59.79 | Size_R_2 | 21 | PRODUCT Size | 184 bp | Primer Start | 54560 | Primer End | 54743 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCACTCTGTTACATGCGACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTGCGTTTCATGCGGAGTG | |||||||||||||
| Tm_F_3 | 60.338 | Size_F_3 | 21 | Tm_R_3 | 59.831 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 54642 | Primer End | 54877 |