Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(T)13
Start:22281 End:22293
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCACGATAATCCCACCCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCCCCAACCTCTGACCAA | |||||||||||||
| Tm_F_1 | 59.89 | Size_F_1 | 20 | Tm_R_1 | 60.105 | Size_R_1 | 20 | PRODUCT Size | 186 bp | Primer Start | 22144 | Primer End | 22329 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCACGATAATCCCACCCCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCTCCCCAACCTCTGACCA | |||||||||||||
| Tm_F_2 | 59.89 | Size_F_2 | 20 | Tm_R_2 | 59.881 | Size_R_2 | 20 | PRODUCT Size | 187 bp | Primer Start | 22144 | Primer End | 22330 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGTTTCCTCTCATCCGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCCCCAACCTCTGACCAA | |||||||||||||
| Tm_F_3 | 59.749 | Size_F_3 | 20 | Tm_R_3 | 60.105 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 22070 | Primer End | 22329 |