Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(T)12
Start:139936 End:139947
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGTGTCCAAAACTGGAATTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCAAGTGGATCAAGGCAA | |||||||||||||
| Tm_F_1 | 59.379 | Size_F_1 | 22 | Tm_R_1 | 59.887 | Size_R_1 | 20 | PRODUCT Size | 202 bp | Primer Start | 139860 | Primer End | 140061 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGTGTCCAAAACTGGAATTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTCACGTTCAGGGAGCCAA | |||||||||||||
| Tm_F_2 | 59.379 | Size_F_2 | 22 | Tm_R_2 | 59.818 | Size_R_2 | 20 | PRODUCT Size | 242 bp | Primer Start | 139860 | Primer End | 140101 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGTGTCCAAAACTGGAATTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCACGTTCAGGGAGCCAAG | |||||||||||||
| Tm_F_3 | 59.379 | Size_F_3 | 22 | Tm_R_3 | 60.25 | Size_R_3 | 20 | PRODUCT Size | 241 bp | Primer Start | 139860 | Primer End | 140100 |