Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(G)12tagggggctgattcaatctaatcaattaatactgtcgttgaacctacttgcgcgtattttgaaagaagt(A)13
Start:31694 End:31787
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTTTCCATCCCTCCCCCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCGGGGAGCGTTTCATTT | |||||||||||||
| Tm_F_1 | 59.877 | Size_F_1 | 20 | Tm_R_1 | 60.034 | Size_R_1 | 20 | PRODUCT Size | 209 bp | Primer Start | 31670 | Primer End | 31878 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCTTTCCATCCCTCCCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCCGGGGAGCGTTTCATTT | |||||||||||||
| Tm_F_2 | 59.648 | Size_F_2 | 20 | Tm_R_2 | 60.034 | Size_R_2 | 20 | PRODUCT Size | 210 bp | Primer Start | 31669 | Primer End | 31878 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATCTTTCCATCCCTCCCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCCGGGGAGCGTTTCATTT | |||||||||||||
| Tm_F_3 | 59.52 | Size_F_3 | 20 | Tm_R_3 | 60.034 | Size_R_3 | 20 | PRODUCT Size | 211 bp | Primer Start | 31668 | Primer End | 31878 |