Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(CCTC)3
Start:82063 End:82074
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TATTGAACCCACGGGCGAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAGTCAGCTGGCACTAACA | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 60.038 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 81858 | Primer End | 82118 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGATTCAACGAAGCTTGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAGTCAGCTGGCACTAACA | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 60.038 | Size_R_2 | 20 | PRODUCT Size | 195 bp | Primer Start | 81924 | Primer End | 82118 |
| FORWARD_PRIMER_3 | |||||||||||||
| TATTGAACCCACGGGCGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGAGTCAGCTGGCACTAAC | |||||||||||||
| Tm_F_3 | 59.964 | Size_F_3 | 20 | Tm_R_3 | 59.756 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 81858 | Primer End | 82119 |