Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(C)15
Start:132548 End:132562
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTTCTAGGAAGGGGGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGCCTCTGAATCTGCAAGA | |||||||||||||
| Tm_F_1 | 59.283 | Size_F_1 | 20 | Tm_R_1 | 60.108 | Size_R_1 | 20 | PRODUCT Size | 242 bp | Primer Start | 132447 | Primer End | 132688 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCTTCTAGGAAGGGGGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGAGCCGCCTCTGAATCTG | |||||||||||||
| Tm_F_2 | 59.283 | Size_F_2 | 20 | Tm_R_2 | 60.108 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 132447 | Primer End | 132693 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCTTCTAGGAAGGGGGAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTTCTTTGAGCCGCCTCT | |||||||||||||
| Tm_F_3 | 59.283 | Size_F_3 | 20 | Tm_R_3 | 59.891 | Size_R_3 | 20 | PRODUCT Size | 254 bp | Primer Start | 132447 | Primer End | 132700 |