Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(C)13
Start:129616 End:129628
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGACCCGCTATTTTGACCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTTTCTCCCCTGGGTGCAT | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.96 | Size_R_1 | 20 | PRODUCT Size | 256 bp | Primer Start | 129533 | Primer End | 129788 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGGACCCGCTATTTTGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTTTCTCCCCTGGGTGCAT | |||||||||||||
| Tm_F_2 | 59.745 | Size_F_2 | 20 | Tm_R_2 | 59.96 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 129531 | Primer End | 129788 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACCCAAGCCAGTGACAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTTTCTCCCCTGGGTGCAT | |||||||||||||
| Tm_F_3 | 59.743 | Size_F_3 | 20 | Tm_R_3 | 59.96 | Size_R_3 | 20 | PRODUCT Size | 243 bp | Primer Start | 129546 | Primer End | 129788 |