Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(C)12tccttaaaagagaagaaggagatagaataaagagaattgaatcctgaatcaatctcttaggtataaattaatcctcccgagacagactca(AT)8
Start:8126 End:8243
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTACGCAGTGGAGTTAAATCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGCTATTGCACTGTACCGT | |||||||||||||
| Tm_F_1 | 59.232 | Size_F_1 | 23 | Tm_R_1 | 59.389 | Size_R_1 | 20 | PRODUCT Size | 256 bp | Primer Start | 8068 | Primer End | 8323 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTACGCAGTGGAGTTAAATCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGCTATTGCACTGTACCGT | |||||||||||||
| Tm_F_2 | 57.881 | Size_F_2 | 22 | Tm_R_2 | 59.389 | Size_R_2 | 20 | PRODUCT Size | 256 bp | Primer Start | 8068 | Primer End | 8323 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTACGCAGTGGAGTTAAATCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGCTATTGCACTGTACCGT | |||||||||||||
| Tm_F_3 | 57.875 | Size_F_3 | 22 | Tm_R_3 | 59.389 | Size_R_3 | 20 | PRODUCT Size | 255 bp | Primer Start | 8069 | Primer End | 8323 |