Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(ATCT)4aa(ATAG)3
Start:33593 End:33622
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGAGGCGTGGCTACCTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTACGTCCCTGGATTCACCG | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 221 bp | Primer Start | 33444 | Primer End | 33664 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAGAGGCGTGGCTACCTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGATTCACCGCATCGAGGTA | |||||||||||||
| Tm_F_2 | 60.036 | Size_F_2 | 20 | Tm_R_2 | 59.612 | Size_R_2 | 20 | PRODUCT Size | 211 bp | Primer Start | 33444 | Primer End | 33654 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGCTACTTGCACCGACGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCAGCCGGGTTTGAAAAAG | |||||||||||||
| Tm_F_3 | 60.388 | Size_F_3 | 20 | Tm_R_3 | 59.896 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 33552 | Primer End | 33831 |