Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(AT)12
Start:70624 End:70647
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGTCGTTAGAAGCATACGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGATACGCCGGTTACGATG | |||||||||||||
| Tm_F_1 | 59.155 | Size_F_1 | 20 | Tm_R_1 | 60.041 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 70514 | Primer End | 70774 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTCGTTAGAAGCATACGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCGATACGCCGGTTACGAT | |||||||||||||
| Tm_F_2 | 59.155 | Size_F_2 | 20 | Tm_R_2 | 59.763 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 70514 | Primer End | 70775 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGTCGTTAGAAGCATACGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTTACCCCTCGATACGCC | |||||||||||||
| Tm_F_3 | 59.155 | Size_F_3 | 20 | Tm_R_3 | 60.25 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 70514 | Primer End | 70784 |