Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(AGAA)3
Start:108199 End:108210
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTAGGGCTACTGGCTGGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAACACCACACGCTAGGTT | |||||||||||||
| Tm_F_1 | 60.473 | Size_F_1 | 20 | Tm_R_1 | 59.243 | Size_R_1 | 20 | PRODUCT Size | 123 bp | Primer Start | 108146 | Primer End | 108268 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCTAGGGCTACTGGCTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAACACCACACGCTAGGTT | |||||||||||||
| Tm_F_2 | 60.473 | Size_F_2 | 20 | Tm_R_2 | 59.243 | Size_R_2 | 20 | PRODUCT Size | 125 bp | Primer Start | 108144 | Primer End | 108268 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCTCGCGATTCGTTCAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTAGAACACCACACGCTAGGT | |||||||||||||
| Tm_F_3 | 59.835 | Size_F_3 | 20 | Tm_R_3 | 59.455 | Size_R_3 | 21 | PRODUCT Size | 101 bp | Primer Start | 108170 | Primer End | 108270 |