Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(AAAG)3
Start:145006 End:145017
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCCCTATGGCATGGGACCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCAGAGCGTGGGATATGGC | |||||||||||||
| Tm_F_1 | 60.104 | Size_F_1 | 20 | Tm_R_1 | 59.893 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 144964 | Primer End | 145200 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCCTATGGCATGGGACCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGAGCGTGGGATATGGCTT | |||||||||||||
| Tm_F_2 | 60.104 | Size_F_2 | 20 | Tm_R_2 | 59.893 | Size_R_2 | 20 | PRODUCT Size | 235 bp | Primer Start | 144964 | Primer End | 145198 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCAAGCACACTGGGTAAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCAGAGCGTGGGATATGGC | |||||||||||||
| Tm_F_3 | 59.686 | Size_F_3 | 20 | Tm_R_3 | 59.893 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 144925 | Primer End | 145200 |