Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)14
Start:48335 End:48348
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGGCTATTCCAACGAACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCGAACGAATGTCGACTCT | |||||||||||||
| Tm_F_1 | 60.035 | Size_F_1 | 20 | Tm_R_1 | 59.76 | Size_R_1 | 20 | PRODUCT Size | 207 bp | Primer Start | 48207 | Primer End | 48413 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGGCTATTCCAACGAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTGCGAACGAATGTCGACT | |||||||||||||
| Tm_F_2 | 60.035 | Size_F_2 | 20 | Tm_R_2 | 59.76 | Size_R_2 | 20 | PRODUCT Size | 209 bp | Primer Start | 48207 | Primer End | 48415 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGGCTATTCCAACGAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGTGATTCTGCGAACGAAT | |||||||||||||
| Tm_F_3 | 60.035 | Size_F_3 | 20 | Tm_R_3 | 59.63 | Size_R_3 | 20 | PRODUCT Size | 216 bp | Primer Start | 48207 | Primer End | 48422 |