Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)13
Start:3443 End:3455
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGTTACCTACTGCTCGCCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGTTCATTCAGGGTCCCTC | |||||||||||||
| Tm_F_1 | 60.109 | Size_F_1 | 20 | Tm_R_1 | 60.108 | Size_R_1 | 20 | PRODUCT Size | 183 bp | Primer Start | 3330 | Primer End | 3512 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTACTGCTCGCCGCTTTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGTTCATTCAGGGTCCCTC | |||||||||||||
| Tm_F_2 | 60.249 | Size_F_2 | 20 | Tm_R_2 | 60.108 | Size_R_2 | 20 | PRODUCT Size | 177 bp | Primer Start | 3336 | Primer End | 3512 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGTTACCTACTGCTCGCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTCATTCAGGGTCCCTCC | |||||||||||||
| Tm_F_3 | 60.109 | Size_F_3 | 20 | Tm_R_3 | 59.744 | Size_R_3 | 20 | PRODUCT Size | 182 bp | Primer Start | 3330 | Primer End | 3511 |