Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)13
Start:153065 End:153077
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATAGCTCGACGCCAGGATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGGTTGGTGAAGATGCGTA | |||||||||||||
| Tm_F_1 | 59.967 | Size_F_1 | 20 | Tm_R_1 | 60.391 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 153031 | Primer End | 153224 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATAGCTCGACGCCAGGATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACGGGTTGGTGAAGATGCG | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 60.391 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 153031 | Primer End | 153226 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGTGGTGAAACTCTACCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGGTTGGTGAAGATGCGTA | |||||||||||||
| Tm_F_3 | 59.966 | Size_F_3 | 20 | Tm_R_3 | 60.391 | Size_R_3 | 20 | PRODUCT Size | 247 bp | Primer Start | 152978 | Primer End | 153224 |