Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)12ttattccttaaaagaatcatccgatagtatctagtatccttcaggtacccagcgataac(T)13
Start:32989 End:33072
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAAACTGGCATGGTGTCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCACAGCCGAATCCCAGAAT | |||||||||||||
| Tm_F_1 | 59.049 | Size_F_1 | 20 | Tm_R_1 | 60.107 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 32877 | Primer End | 33147 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACTGGCATGGTGTCGAAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCACAGCCGAATCCCAGAAT | |||||||||||||
| Tm_F_2 | 59.048 | Size_F_2 | 20 | Tm_R_2 | 60.107 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 32881 | Primer End | 33147 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGGTTCGAATCCCTCTCTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATACTGATGCGGGTCCCAG | |||||||||||||
| Tm_F_3 | 59.71 | Size_F_3 | 21 | Tm_R_3 | 59.966 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 32908 | Primer End | 33167 |