Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)12
Start:85654 End:85665
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTCTCGGGTTCGTACCTCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTACGCCGCAAAAGAATGA | |||||||||||||
| Tm_F_1 | 59.902 | Size_F_1 | 20 | Tm_R_1 | 59.559 | Size_R_1 | 20 | PRODUCT Size | 225 bp | Primer Start | 85510 | Primer End | 85734 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTCTCGGGTTCGTACCTCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGTACGCCGCAAAAGAATG | |||||||||||||
| Tm_F_2 | 59.902 | Size_F_2 | 20 | Tm_R_2 | 60.52 | Size_R_2 | 20 | PRODUCT Size | 226 bp | Primer Start | 85510 | Primer End | 85735 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTCTCGGGTTCGTACCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTACGCCGCAAAAGAATGA | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 59.559 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 85509 | Primer End | 85734 |