Primers Available
Organism: Alsophila spinulosa chloroplast
Repeat:(A)12
Start:28198 End:28209
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGGCCTCTAGACGATGGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATCAATCAGCCGCTGCAAC | |||||||||||||
| Tm_F_1 | 59.887 | Size_F_1 | 20 | Tm_R_1 | 59.828 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 28113 | Primer End | 28328 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAGGGGAATTCGAATCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATCAATCAGCCGCTGCAAC | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.828 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 28067 | Primer End | 28328 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCAGGGGAATTCGAATCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATCAATCAGCCGCTGCAAC | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 59.828 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 28066 | Primer End | 28328 |