Primers Available
Organism: Thermophis zhaoermii mitochondrion
Repeat:(CAA)4
Start:5132 End:5143
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACAGCCCTACTATTCGCTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAAAGGGTGCAGCTGCTAT | |||||||||||||
| Tm_F_1 | 59.895 | Size_F_1 | 20 | Tm_R_1 | 59.671 | Size_R_1 | 20 | PRODUCT Size | 134 bp | Primer Start | 5058 | Primer End | 5191 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTACTATTCGCTGCCACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAAAGGGTGCAGCTGCTAT | |||||||||||||
| Tm_F_2 | 60.25 | Size_F_2 | 20 | Tm_R_2 | 59.671 | Size_R_2 | 20 | PRODUCT Size | 130 bp | Primer Start | 5062 | Primer End | 5191 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGCGAACGCTATAAACACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAAGGGTGCAGCTGCTAT | |||||||||||||
| Tm_F_3 | 59.7 | Size_F_3 | 20 | Tm_R_3 | 59.671 | Size_R_3 | 20 | PRODUCT Size | 112 bp | Primer Start | 5080 | Primer End | 5191 |