Primers Available
Organism: Urechis unicinctus mitochondrion
Repeat:(AAT)4
Start:6843 End:6854
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTGCGCCAGATGAATGGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCGTTTTGTGTGGGTCAAA | |||||||||||||
| Tm_F_1 | 59.818 | Size_F_1 | 20 | Tm_R_1 | 59.826 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 6708 | Primer End | 6982 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGCCTTTCGCTCTAGGGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAAACACTGCGGGAGGTAA | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 60.251 | Size_R_2 | 20 | PRODUCT Size | 252 bp | Primer Start | 6644 | Primer End | 6895 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTGCGCCAGATGAATGGAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAAACACTGCGGGAGGTAA | |||||||||||||
| Tm_F_3 | 59.818 | Size_F_3 | 20 | Tm_R_3 | 60.251 | Size_R_3 | 20 | PRODUCT Size | 188 bp | Primer Start | 6708 | Primer End | 6895 |