Primers Available
Organism: Crassostrea sikamea mitochondrion
Repeat:(TTTTG)3
Start:3316 End:3330
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGCGTGTATGGCAGATGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCCCATAAAACTCTGGCACA | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 59.925 | Size_R_1 | 21 | PRODUCT Size | 243 bp | Primer Start | 3241 | Primer End | 3483 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCTGATGGCGTGTATGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCCCATAAAACTCTGGCACA | |||||||||||||
| Tm_F_2 | 59.895 | Size_F_2 | 20 | Tm_R_2 | 59.925 | Size_R_2 | 21 | PRODUCT Size | 250 bp | Primer Start | 3234 | Primer End | 3483 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGCTGACAGGCCTTCATGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCCCATAAAACTCTGGCACA | |||||||||||||
| Tm_F_3 | 60.395 | Size_F_3 | 20 | Tm_R_3 | 59.925 | Size_R_3 | 21 | PRODUCT Size | 218 bp | Primer Start | 3266 | Primer End | 3483 |