Primers Available
Organism: Ascobulla fragilis mitochondrion
Repeat:(TGTT)3
Start:4063 End:4074
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGAATCCCTAAGGGCAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGCCCTGACAGGAGTTGG | |||||||||||||
| Tm_F_1 | 60.326 | Size_F_1 | 20 | Tm_R_1 | 59.889 | Size_R_1 | 20 | PRODUCT Size | 244 bp | Primer Start | 3988 | Primer End | 4231 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGAATCCCTAAGGGCAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCCGGCAAAAGCCAAAAG | |||||||||||||
| Tm_F_2 | 60.326 | Size_F_2 | 20 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 169 bp | Primer Start | 3988 | Primer End | 4156 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGAATCCCTAAGGGCAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAGTTGGAGCCGCTATAGC | |||||||||||||
| Tm_F_3 | 60.326 | Size_F_3 | 20 | Tm_R_3 | 60.32 | Size_R_3 | 20 | PRODUCT Size | 232 bp | Primer Start | 3988 | Primer End | 4219 |