Primers Available
Organism: Ascobulla fragilis mitochondrion
Repeat:(AAAC)3
Start:9474 End:9485
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGGGTAAAATCAGCTTCGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGACCTCTCACTTTGACTGT | |||||||||||||
| Tm_F_1 | 58.64 | Size_F_1 | 21 | Tm_R_1 | 58.692 | Size_R_1 | 21 | PRODUCT Size | 254 bp | Primer Start | 9381 | Primer End | 9634 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGGTAAAATCAGCTTCGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGACCTCTCACTTTGACTGT | |||||||||||||
| Tm_F_2 | 57.51 | Size_F_2 | 20 | Tm_R_2 | 58.692 | Size_R_2 | 21 | PRODUCT Size | 253 bp | Primer Start | 9382 | Primer End | 9634 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGGGTAAAATCAGCTTCGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAGGACACATTGTTATAGGCC | |||||||||||||
| Tm_F_3 | 58.64 | Size_F_3 | 21 | Tm_R_3 | 59.381 | Size_R_3 | 22 | PRODUCT Size | 211 bp | Primer Start | 9381 | Primer End | 9591 |