Primers Available
Organism: Sipunculus nudus mitochondrion
Repeat:(CCTC)3
Start:9869 End:9880
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAGGCAGGCACCTACTTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGTGCTAAGGTGTCCGGTT | |||||||||||||
| Tm_F_1 | 60.253 | Size_F_1 | 20 | Tm_R_1 | 59.965 | Size_R_1 | 20 | PRODUCT Size | 102 bp | Primer Start | 9829 | Primer End | 9930 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAGGCAGGCACCTACTTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGGAAACTAGTTGGGGCCC | |||||||||||||
| Tm_F_2 | 60.253 | Size_F_2 | 20 | Tm_R_2 | 59.886 | Size_R_2 | 20 | PRODUCT Size | 139 bp | Primer Start | 9829 | Primer End | 9967 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCAGGCAGGCACCTACTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGATTATTGACCCGGCCACG | |||||||||||||
| Tm_F_3 | 60.253 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 264 bp | Primer Start | 9829 | Primer End | 10092 |