Primers Available
Organism: Sphaerius sp. BT0074 mitochondrion
Repeat:(TAT)5
Start:655 End:669
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATAGGAAGCGCCCCATTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTTTAAACCTCCAATTCTTCCAA | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 57.586 | Size_R_1 | 24 | PRODUCT Size | 188 bp | Primer Start | 516 | Primer End | 703 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGCCCCATTCCATTTTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTTAAACCTCCAATTCTTCCAA | |||||||||||||
| Tm_F_2 | 59.751 | Size_F_2 | 20 | Tm_R_2 | 57.586 | Size_R_2 | 24 | PRODUCT Size | 180 bp | Primer Start | 524 | Primer End | 703 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCGCCCCATTCCATTTTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTTAAACCTCCAATTCTTCCAA | |||||||||||||
| Tm_F_3 | 59.75 | Size_F_3 | 20 | Tm_R_3 | 57.586 | Size_R_3 | 24 | PRODUCT Size | 181 bp | Primer Start | 523 | Primer End | 703 |