Primers Available
Organism: Ascaloptynx appendiculatus mitochondrion
Repeat:(ATA)4
Start:5625 End:5636
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCAGCAGCATGATACTGACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCACATTCAAATGGAGAGTTTTT | |||||||||||||
| Tm_F_1 | 59.791 | Size_F_1 | 21 | Tm_R_1 | 57.27 | Size_R_1 | 24 | PRODUCT Size | 274 bp | Primer Start | 5425 | Primer End | 5698 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAGCAGCATGATACTGACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCACATTCAAATGGAGAGTTTTT | |||||||||||||
| Tm_F_2 | 58.34 | Size_F_2 | 20 | Tm_R_2 | 57.27 | Size_R_2 | 24 | PRODUCT Size | 273 bp | Primer Start | 5426 | Primer End | 5698 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTTGACTTCCAATCAAAAGGTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGCTGAGCATTTTGGATCA | |||||||||||||
| Tm_F_3 | 57.016 | Size_F_3 | 24 | Tm_R_3 | 59.53 | Size_R_3 | 21 | PRODUCT Size | 193 bp | Primer Start | 5529 | Primer End | 5721 |