Primers Available
Organism:Blastocystis sp. DMP/02-328 mitochondrion
Repeat:(T)15aattgcaa(T)13
Start:19703 End:19738
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGTAATAAATGCTGATAAAAATGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCAAAACCCTTAAATACGAAA | |||||||||||||
| Tm_F_1 | 60.008 | Size_F_1 | 25 | Tm_R_1 | 58.718 | Size_R_1 | 22 | PRODUCT Size | 279 bp | Primer Start | 19568 | Primer End | 19846 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGGTAATAAATGCTGATAAAAATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCAAAACCCTTAAATACGAAA | |||||||||||||
| Tm_F_2 | 60.008 | Size_F_2 | 25 | Tm_R_2 | 58.718 | Size_R_2 | 22 | PRODUCT Size | 280 bp | Primer Start | 19567 | Primer End | 19846 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCAATTTTTGTTGAACCTTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTATGATTTAACTCCCATTCCA | |||||||||||||
| Tm_F_3 | 59.911 | Size_F_3 | 23 | Tm_R_3 | 57.592 | Size_R_3 | 23 | PRODUCT Size | 252 bp | Primer Start | 19521 | Primer End | 19772 |