Primers Available
Organism: Rhodeus ocellatus mitochondrion
Repeat:(ATT)5
Start:9667 End:9681
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCGCCTGATACTGACACTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCACTCGTAGGGTGAAAGCT | |||||||||||||
| Tm_F_1 | 60.108 | Size_F_1 | 20 | Tm_R_1 | 60.037 | Size_R_1 | 20 | PRODUCT Size | 264 bp | Primer Start | 9507 | Primer End | 9770 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCGCCTGATACTGACACTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGGGTCATCTGTGGAAGTC | |||||||||||||
| Tm_F_2 | 60.108 | Size_F_2 | 20 | Tm_R_2 | 60.108 | Size_R_2 | 20 | PRODUCT Size | 234 bp | Primer Start | 9507 | Primer End | 9740 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCGCCTGATACTGACACTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAAGCTTTTCTGCGTCCGG | |||||||||||||
| Tm_F_3 | 60.108 | Size_F_3 | 20 | Tm_R_3 | 60.11 | Size_R_3 | 20 | PRODUCT Size | 251 bp | Primer Start | 9507 | Primer End | 9757 |