Primers Available
Organism: Pinus monophylla chloroplast
Repeat:(ATTGAT)4
Start:28376 End:28399
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGCGAGTGCTAGGTCGATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGCTGGTTCAAATCCAGCT | |||||||||||||
| Tm_F_1 | 59.969 | Size_F_1 | 20 | Tm_R_1 | 60.251 | Size_R_1 | 20 | PRODUCT Size | 226 bp | Primer Start | 28209 | Primer End | 28434 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGCGAGTGCTAGGTCGATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCCCGAGTGGTTAATGAGG | |||||||||||||
| Tm_F_2 | 59.969 | Size_F_2 | 20 | Tm_R_2 | 59.748 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 28209 | Primer End | 28484 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCGAGTGCTAGGTCGATGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGCTGGTTCAAATCCAGCT | |||||||||||||
| Tm_F_3 | 59.899 | Size_F_3 | 20 | Tm_R_3 | 60.251 | Size_R_3 | 20 | PRODUCT Size | 224 bp | Primer Start | 28211 | Primer End | 28434 |