Primers Available
Organism: Sagamichthys abei mitochondrion
Repeat:(CCTC)4
Start:5764 End:5779
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGCTTTGGAAACTGGCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCCCAGGATTGAGGAGACA | |||||||||||||
| Tm_F_1 | 60.252 | Size_F_1 | 20 | Tm_R_1 | 59.881 | Size_R_1 | 20 | PRODUCT Size | 255 bp | Primer Start | 5678 | Primer End | 5932 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGCTTTGGAAACTGGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGGATTGAGGAGACACCCG | |||||||||||||
| Tm_F_2 | 60.252 | Size_F_2 | 20 | Tm_R_2 | 59.823 | Size_R_2 | 20 | PRODUCT Size | 251 bp | Primer Start | 5678 | Primer End | 5928 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGAGCTGAACTAAGCCAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACGGTTCATCCTGTTCCGG | |||||||||||||
| Tm_F_3 | 60.109 | Size_F_3 | 20 | Tm_R_3 | 60.321 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 5562 | Primer End | 5835 |