Primers Available
Organism: Rhagophthalmus lufengensis mitochondrion
Repeat:(ATT)4
Start:4356 End:4367
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGCCCAGGAACTTTAGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAATTGGTCATGGACTTACTTCT | |||||||||||||
| Tm_F_1 | 59.309 | Size_F_1 | 20 | Tm_R_1 | 57.62 | Size_R_1 | 24 | PRODUCT Size | 279 bp | Primer Start | 4228 | Primer End | 4506 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCGCCCAGGAACTTTAGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGAATTGGTCATGGACTTACTTCT | |||||||||||||
| Tm_F_2 | 59.309 | Size_F_2 | 20 | Tm_R_2 | 58.216 | Size_R_2 | 25 | PRODUCT Size | 280 bp | Primer Start | 4228 | Primer End | 4507 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCTGTAGGAACACCACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGTATGATTGAATAATTGCTACTGCT | |||||||||||||
| Tm_F_3 | 60.105 | Size_F_3 | 20 | Tm_R_3 | 59.558 | Size_R_3 | 27 | PRODUCT Size | 244 bp | Primer Start | 4162 | Primer End | 4405 |