Primers Available
Organism: Heterosigma akashiwo chloroplast
Repeat:(AAAG)3
Start:60411 End:60422
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCAAACTACGCTACATCCCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGGGACTGACCAAAAATGCT | |||||||||||||
| Tm_F_1 | 60.135 | Size_F_1 | 21 | Tm_R_1 | 60.133 | Size_R_1 | 21 | PRODUCT Size | 264 bp | Primer Start | 60253 | Primer End | 60516 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAACTACGCTACATCCCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGGGACTGACCAAAAATGCT | |||||||||||||
| Tm_F_2 | 59.864 | Size_F_2 | 21 | Tm_R_2 | 60.133 | Size_R_2 | 21 | PRODUCT Size | 263 bp | Primer Start | 60254 | Primer End | 60516 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCAAACTACGCTACATCCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGGAATTGGGGACTGACCA | |||||||||||||
| Tm_F_3 | 60.135 | Size_F_3 | 21 | Tm_R_3 | 58.846 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 60253 | Primer End | 60523 |