Primers Available
Organism:Dictyostelium fasciculatum mitochondrion
Repeat:(ATTA)3
Start:53690 End:53701
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCATTAAGAAGACCTCCAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAATTTTGATTCATTTTTGATTCC | |||||||||||||
| Tm_F_1 | 60.222 | Size_F_1 | 21 | Tm_R_1 | 58.798 | Size_R_1 | 25 | PRODUCT Size | 239 bp | Primer Start | 53500 | Primer End | 53738 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCAAAGTCATACTGTTGTTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTGTTTCTCTATTTGAACCCTT | |||||||||||||
| Tm_F_2 | 59.54 | Size_F_2 | 22 | Tm_R_2 | 59.02 | Size_R_2 | 24 | PRODUCT Size | 235 bp | Primer Start | 53628 | Primer End | 53862 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAAAGTCATACTGTTGTTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTGTTTCTCTATTTGAACCCTT | |||||||||||||
| Tm_F_3 | 59.54 | Size_F_3 | 22 | Tm_R_3 | 59.02 | Size_R_3 | 24 | PRODUCT Size | 234 bp | Primer Start | 53629 | Primer End | 53862 |