Primers Available
Organism:Dictyostelium fasciculatum mitochondrion
Repeat:(ATT)4
Start:6647 End:6658
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGGTTTTTGATTATTGCCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCCATTTGATCACGATAGA | |||||||||||||
| Tm_F_1 | 59.832 | Size_F_1 | 20 | Tm_R_1 | 59.646 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 6539 | Primer End | 6808 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGGTTTTTGATTATTGCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAATCGCCATTTGATCACG | |||||||||||||
| Tm_F_2 | 59.832 | Size_F_2 | 20 | Tm_R_2 | 60.837 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 6539 | Primer End | 6813 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGTTTTTGATTATTGCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATCGCCATTTGATCACGA | |||||||||||||
| Tm_F_3 | 59.832 | Size_F_3 | 20 | Tm_R_3 | 60.025 | Size_R_3 | 19 | PRODUCT Size | 273 bp | Primer Start | 6539 | Primer End | 6811 |