Primers Available
Organism:Dictyostelium fasciculatum mitochondrion
Repeat:(A)10
Start:43696 End:43705
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCGGGTATATTTAAGAAAAGTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACCAAAACGATTATGACAGC | |||||||||||||
| Tm_F_1 | 59.139 | Size_F_1 | 24 | Tm_R_1 | 58.188 | Size_R_1 | 22 | PRODUCT Size | 273 bp | Primer Start | 43500 | Primer End | 43772 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGGGTATATTTAAGAAAAGTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCAAAACGATTATGACAGCTT | |||||||||||||
| Tm_F_2 | 59.139 | Size_F_2 | 24 | Tm_R_2 | 59.113 | Size_R_2 | 23 | PRODUCT Size | 272 bp | Primer Start | 43500 | Primer End | 43771 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTAAACAAGTAAAGCAAGAGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCGTTGTAACTGGCTATT | |||||||||||||
| Tm_F_3 | 57.337 | Size_F_3 | 26 | Tm_R_3 | 60.152 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 43592 | Primer End | 43871 |