Primers Available
Organism: Brachionus plicatilis mitochondrion part II
Repeat:(TGAT)3
Start:9709 End:9720
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTAACCCCCACGTTGTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGCCCTATGGATCAAGTCT | |||||||||||||
| Tm_F_1 | 59.963 | Size_F_1 | 20 | Tm_R_1 | 57.912 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 9556 | Primer End | 9828 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCTAACCCCCACGTTGTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGCTAATCGCCCTATGGATCA | |||||||||||||
| Tm_F_2 | 59.963 | Size_F_2 | 20 | Tm_R_2 | 59.502 | Size_R_2 | 22 | PRODUCT Size | 280 bp | Primer Start | 9556 | Primer End | 9835 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGACTCTAACTGGACCTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAAGAGCTAATCGCCCTATGG | |||||||||||||
| Tm_F_3 | 59.462 | Size_F_3 | 20 | Tm_R_3 | 59.962 | Size_R_3 | 22 | PRODUCT Size | 197 bp | Primer Start | 9643 | Primer End | 9839 |