Primers Available
Organism: Brachionus plicatilis mitochondrion part I
Repeat:(TGAT)3
Start:3237 End:3248
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTAACCCCCACGTTGTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGCCCTATGGATCAAGTCT | |||||||||||||
| Tm_F_1 | 59.963 | Size_F_1 | 20 | Tm_R_1 | 57.912 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 3084 | Primer End | 3356 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCTAACCCCCACGTTGTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGCTAATCGCCCTATGGATCA | |||||||||||||
| Tm_F_2 | 59.963 | Size_F_2 | 20 | Tm_R_2 | 59.502 | Size_R_2 | 22 | PRODUCT Size | 280 bp | Primer Start | 3084 | Primer End | 3363 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGACTCTAACTGGACCTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAAGAGCTAATCGCCCTATGG | |||||||||||||
| Tm_F_3 | 59.462 | Size_F_3 | 20 | Tm_R_3 | 59.962 | Size_R_3 | 22 | PRODUCT Size | 197 bp | Primer Start | 3171 | Primer End | 3367 |