Primers Available
Organism: Manihot esculenta chloroplast
Repeat:(T)18c(A)15
Start:12784 End:12817
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATGCCGTCGCCTACTTGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCATGTTTGGCGCGATAAA | |||||||||||||
| Tm_F_1 | 60.037 | Size_F_1 | 20 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 223 bp | Primer Start | 12650 | Primer End | 12872 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCGTCGGCTCGAATGGTTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCATGTTTGGCGCGATAAA | |||||||||||||
| Tm_F_2 | 59.9 | Size_F_2 | 20 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 130 bp | Primer Start | 12743 | Primer End | 12872 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTCGGCTCGAATGGTTACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCATGTTTGGCGCGATAAA | |||||||||||||
| Tm_F_3 | 60.109 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 127 bp | Primer Start | 12746 | Primer End | 12872 |